Anti-Interferon gamma (IFNG) (IFNG/466)

Anti-Interferon gamma (IFNG) (IFNG/466)

• Description: This antibody recognizes a protein of 20-25 kDa, identified as human interferon. This MAb is specific to human IFN-γ and recognizes both recombinant and native human IFN-gamma. T lymphocytes and NK cells mainly produce IFN-γ. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses, including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak anti-viral and anti-proliferative activity, and potentiates the antiviral and anti-tumor effects of IFN-γ (type I interferon).Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: AMCF1; Beta thromboglobulin like protein; CXC chemokine ligand 8 (CXCL8); Emoctakin; Granulocyte chemotactic protein 1 (GCP1); IFG; IFI; IFN Immune; Interleukin 8; LECT; LUCT; Lymphocyte derived neutrophil activating factor; LYNAP; Macrophage Activating Factor (MAF); Monocyte derived neutrophil activating protein (MONAP); Monocyte derived neutrophil chemotactic factor (MDNCF); Neutrophil activating factor (NAF); Neutrophil activating peptide 1 (NAP1); SCYB8
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: IFNG
• Gene ID: 3458
• NCBI Gene ID: 856
• UniProt: P01579
• Cellular Locus: Secreted (extracellular)
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Purified recombinant human IFN-gamma
• Target Antigen: Interferon Gamma
• Clonality: Monoclonal
• Isotype: IgG2b κ
• Clone: IFNG/466
• Conjugation: Purified, with BSA
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Field of Research: Cytokines, Immunology, Inflammation
• Positive Control: PMA stimulated human peripheral blood mononuclear cells. Renal or testicular carcinoma.
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: 20-25 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology (formalin) 1-2 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years