Goat anti-Rab8 Polyclonal antibody

• Description:

  Goat polyclonal antibody to mouse Rab8. Rab8 belongs to the small GTPase superfamily, Rab family. It has cell-type specific function during the process of exocytosis and coordinates regulation of the cytoskeleton. Rab8 also appears to interface with endocytic recycling circuits. At the molecular level, this GTPase regulates both the export of vesicles from the trans-Golgi apparatus, and the directed translocation along actin filaments and microtubules.

• Specifications:

  Detects total Rab8 protein in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab8a and GFP-Rab8b cds by Western blot.

• Product Name Alternative:

  RAB8A, RAB8B, RAS-associated protein RAB8A, ras-related protein Rab-8B, ras-related protein Rab-8A, ras-related protein Rab-8B antibody.

• UNSPSC Description:

  RAB8A, member RAS oncogene family

• Volume:

  200 µL

• Gene ID:

  ENSG00000167461

• Accession Number:

  ENSG00000167461

• Host:

  Goat

• Antigen Species:

  Mouse

• Reactivity:

  Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep, simian, other

• Immunogen:

  Purified recombinant peptide derived from within residues 107 aa to the C-terminus of mouse Rab8a and Rab8b produced in E. coli.

• Target Antigen:

  Purified recombinant peptide derived from within residues 107 aa to the C-terminus of mouse Rab8a and Rab8b produced in E. coli.

• Immunogen Type:

  Recombinant protein

• Target:

  Anti-Rab8

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Type:

  Primary

• Applications:

  WB, IF, IHC-P, IHC-F

• Purification:

  Epitope affinity purified

• Concentration:

  3 mg/mL

• Dilution:

  WB:1:250-1:1,000, IF:1:50-1:250, IHC-P:1:250-1:500, IHC-F:1:250-1:500

• Form:

  ​Polyclonal antibody supplied as a 200 µl (3 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

• Buffer:

  PBS, 20% glycerol and 0.05% sodium azide

• References & Citations:

  1. Schiweck J, Murk K, Ledderose J, et al. Nature Communications, 2021. PMID: 33674568, 2. Schiweck J, Murk K, Ledderose J, et al. bioRxiv 2020

• Storage Conditions:

  For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.

• CAS Number:

  9007-83-4