Anti-CD31 / PECAM-1 (Endothelial Cell Marker) (C31/1395R), Biotin conjugate

• Description:

  CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA'

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  PECAM1

• Gene ID:

  5175

• NCBI Gene ID:

  376675 & 514412

• UniProt:

  P16284

• Cellular Locus:

  Plasma membrane

• Host:

  Rabbit

• Species Reactivity:

  Human

• Immunogen:

  Human recombinant full-length CD31 protein

• Target Antigen:

  CD31 | PECAM-1

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG κ

• Clone:

  C31/1395R

• Conjugation:

  Biotin

• Source:

  Animal

• Applications:

  Flow (verified) | IHC, FFPE (verified) | WB (verified)

• Validated Applications:

  FC, IHC, FFPE, WB

• Field of Research:

  Angiogenesis, Cancer

• Positive Control:

  Jurkat cells. Tonsil or Angiosarcoma.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  ~100 kDa (endothelium)|~130 kDa (platelets)

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology (formalin) 1-2 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4