Anti-CD44v4 (Marker of Tumor Metastasis) (CD44v4/1700R), CF594 conjugate

Anti-CD44v4 (Marker of Tumor Metastasis) (CD44v4/1700R), CF594 conjugate

• Description: This antibody recognizes an epitope encoded by exon v4 on the variant portion of human CD44. The CD44 molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. CD44 is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA. The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: LHR; BA-1; chondroitin sulfate proteoglycan 8 (CSPG8); Epican; Extracellular Matrix Receptor III (ECM III); GP90 Lymphocyte Homing Adhesion Receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan Sulfate Proteoglycan; Hermes Antigen; HAS; HUTCH I; Hyaluronate Receptor; Indian blood group; Inlu Related p80 Glycoprotein; Ly 24; MDU2; MDU3; MIC4; MUTCH I; Phagocytic Glycoprotein 1 (PGP-1)
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CD44
• Gene ID: 960
• NCBI Gene ID: 502328
• UniProt: P16070
• Cellular Locus: Plasma membrane
• Host: Rabbit
• Species Reactivity: Human
• Immunogen: Recombinant fragment corresponding to the v4 domain of human CD44 (exact sequence is proprietary)
• Target Antigen: CD44v4
• Clonality: Recombinant Monoclonal
• Isotype: IgG κ
• Clone: CD44v4/1700R
• Conjugation: CF594
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Field of Research: Cancer, Immunology
• Positive Control: Cervix or Tongue Squamous Cell Carcinoma
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 80-95 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology (formalin): 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years