Anti-Calnexin (Endoplasmic Reticulum Marker) (CANX/1541), CF594 conjugate

• Description:

  Calnexin is a type-I integral membrane protein of the endoplasmic reticulum (ER). It is a calcium-binding chaperone protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation._x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

• Synonyms:

  Calnexin; CANX; IP90; Major histocompatibility complex class I antigen-binding protein p88

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CANX

• Gene ID:

  821

• NCBI Gene ID:

  567968

• UniProt:

  P27824

• Cellular Locus:

  Endoplasmic reticulum

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant N-terminal fragment of human Calnexin protein (exact sequence is proprietary)

• Target Antigen:

  Calnexin

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  CANX/1541

• Conjugation:

  CF594

• Source:

  Animal

• Applications:

  Flow, intracellular | IF (verified) | IHC, FFPE (verified) | WB (verified)

• Validated Applications:

  FC, IF, IHC, FFPE, WB

• Field of Research:

  Chaperones, Immunology

• Positive Control:

  HeLa, MCF-7 or U2OS cells. Kidney or small intestine.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  67 kDa (predicted); 80-90 kDa (observed)

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology (formalin) 1-2 ug/mL|Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4