Biotinylated Human HLA-A*02:01&B2M&EBV EBNA (FMVFLQTHI) Complex Protein (Monomer, MALS verified)

Biotinylated Human HLA-A*02:01&B2M&EBV EBNA (FMVFLQTHI) Complex Protein (Monomer, MALS verified)

• Description: Biotinylated Human HLA-A*02:01&B2M&EBV EBNA (FMVFLQTHI) Complex Protein (HLA-H82E8) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & FMVFLQTHI peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & FMVFLQTHI).
• Specifications: Biotinylated Human HLA-A*02:01&B2M&EBV EBNA (FMVFLQTHI) Complex Protein is produced by co-expression of HLA and B2M loaded with EBV EBNA peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 36.3 kDa and 11.7 kDa. The protein migrates as 41-43 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & FMVFLQTHI peptide
• Host: HEK 293
• Target: HLA-A*0201 & B2M & EBV EBNA (FMVFLQTHI)
• Conjugation: Biotin-labeled
• Tag: C-10×His & C-Avi
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Bioactivity: Immobilized Anti-HLA class I Antibody, Human IgG1 (W6/32) at 1 μg/mL (100 μL/well) can bind Biotinylated Human HLA-A*02:01&B2M&EBV EBNA (FMVFLQTHI) Complex Protein (Cat. No. HLA-H82E8) with a linear range of 0.5-8 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 36.3 kDa and 11.7 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C