Anti-TSH-Receptor, A-Chain (Thyroid Marker) (TSHRA/1402), CF488A conjugate

• Description:

  Thyroid-stimulating hormone (TSH, also known as thyrotropin) is a glycoprotein involved in the control of thyroid structure and metabolism, which stimulates the release of the thyroid hormones. TSH is regulated by thyroid hormone (T3) and various retinoid compounds. TSH binds to the thyroid-stimulating hormone receptor (TSHR), which is cleaved into two subunits, A and B, and plays a major role in regulating thyroid function. The third cytoplasmic loop of TSHR has been identified as critical for its role in regulating inositol phosphate and cAMP formation. In Graves disease, an autoimmune disorder, TSHR is activated by autoantibodies, which may be stimulated by the cleavage of the A and B subunits.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  CHNG1; hTSHRI; LGR3; Thyroid-stimulating hormone receptor; Thyrotropin receptor; Thyrotropin receptor I; TSHR

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  TSHR

• Gene ID:

  7253

• NCBI Gene ID:

  160411

• UniProt:

  P16473

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  A-Chain protein|Recombinant human TSHR

• Target Antigen:

  TSH Receptor, A Chain

• Clonality:

  Monoclonal

• Isotype:

  IgG κ

• Clone:

  TSHRA/1402

• Conjugation:

  CF488A

• Source:

  Animal

• Applications:

  IHC, FFPE (verified)

• Validated Applications:

  IHC, FFPE

• Field of Research:

  Endocrinology

• Positive Control:

  293T Cells. Thyroid gland

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  62 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology (formalin): 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4