Anti-CD61 / Integrin beta-3 / Platelet Glycoprotein IIIa (Platelet Marker) (ITGB3/1713), CF568 conjugate

• Description:

  This antibody reacts with human integrin beta3 (GPIIIa, vitronectin receptor beta chain). It associates with the alpha-V-chain (CD51) to form vitronectin receptor, or with the alpha-IIb-chain (CD41) to form the GpIIb/GpIIIa complex (CD41/CD61). The CD41/CD61 complex appears early in megakaryocyte maturation. The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen, fibronectin, vitronectin and thrombospondin. It plays a central role in platelet activation and aggregation. The CD51/CD61 is implicated in tumor metastasis and adenoviral infection. The antibody detects platelets in smears of blood and bone marrow, as well as megakaryocytes in frozen sections and cell smears. The antibody is useful for classification of megakaryoblastic leukemia.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  BDPLT2; GP3A; GPIIIa; GT; HPA 1; HPA 4; Integrin beta-3; ITGB3; NAIT; Platelet fibrinogen receptor beta subunit; Platelet glycoprotein IIIa; PTP

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  ITGB3

• Gene ID:

  3690

• NCBI Gene ID:

  218040

• UniProt:

  P05106

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant human ITGB3 protein

• Target Antigen:

  CD61 | Integrin-Beta-3 | Platelet-Glycoprotein-IIIa

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  ITGB3/1713

• Conjugation:

  CF568

• Source:

  Animal

• Applications:

  IHC, FFPE (verified)

• Validated Applications:

  IHC, FFPE

• Field of Research:

  Cell adhesion

• Positive Control:

  U937, KG1a, HEL cells. Spleen

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  105 kDa & 90 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Flow cytometry: 0.5-1 ug/million cells in 0.1mL|Immunofluorescence: 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min|Immunohistology (frozen or formalin) 1-2 ug/mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years