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BKS DB Mouse Small Intestinal Microvascular Endothelial Cells

CAT:
870-ABC-TC5495
Size:
1 Vial
Price:
Ask
For price, please contact [email protected]
  • Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
  • Dry Ice Shipment: Yes
BKS DB Mouse Small Intestinal Microvascular Endothelial Cells - image 1

BKS DB Mouse Small Intestinal Microvascular Endothelial Cells

  • Description: BKS db Mouse Small Intestinal Microvascular Endothelial Cells are isolated from the small intestinal microvascular of Mice homozygous for the diabetes spontaneous mutation (Lepr/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoRIemic. BKS db Mouse Small Intestinal Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in AcceGen's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×106 cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.
  • Applications: For research use only
  • Storage Conditions: Liquid Nitrogen