Products for Research Use Only

RFP-Human Dopaminergic Neuron Cells

CAT: 0870-ABC-FC0046Size: 1 VialDry Ice: YesHazardous: No
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CAT#:0870-ABC-FC0046Size:1 Vial
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Human dopaminergic neuronal cells are isolated from fetal brain tissue obtained from agencies authorized to procure and distribute tissues for research. Dopaminergic neuronal cells are selected in tyrosine free medium supplemented with a mixture of growth factors. Actively growing population of cells are tested for tyrosine hydroxylase (TH) expression by immunocytochemistry. RFP-Expressing Human dopaminergic neuronal cells (RFP-HDaNCs) are selected by puromycin after HDaNCs are infected with lentiviruses expressing RFP. Cells are provided with more than 5x10[5] cells/ vial at passage 2. The cells are grown in regular tissue culture dishes and subcultured at a split ratio of 1 to 2 at confluence. We recommend not to use the cells beyond 4 passages. There may be a significant decrease in the number of tyrosine hydroxylase positive cells after 4 passages as they are not maintained in selective medium to allow the growth of only cells expressing tyrosine hydroxylase.
Applications
For research use only
Storage Conditions
Liquid Nitrogen