HighYield T7 sgRNA Synthesis Kit (SpCas9)

• Description:

  HighYield T7 sgRNA Synthesis Kit (SpCas9) is designed for cloning-free synthesis of SpCas9-specific single-guided RNAs (sgRNAs) via in vitro transcription. SgRNAs direct sequence-specific DNA recognition once complexed with Streptococcus pyogenes Cas9 (SpCas9)[1,2]. The resulting sgRNA/SpCas9 ribonucleoprotein (RNP) complex can thus be used for site-specific cleavage, nicking or binding of dsDNA both in vitro and in living cells depending on the choice of SpCas9 variant (e.g. wildtype, nickase (D10A), nuclease deficient(D10A/H840A)). Cleavage, nicking or binding of SpCas9 variant occurs upstream of the SpCas9-specific DNA recognition sequence 5'-NGG-3' (protospacer adjacent motif (PAM) sequence, N = any nucleotide base).

• Product Name Alternative:

  Cloning-free preparation of SpCas9-specific sgRNA by in vitro transcription

• Synonyms:

  Cloning-free preparation of SpCas9-specific sgRNA by in vitro transcription

• UNSPSC:

  41100000

• UNSPSC Description:

  Laboratory and scientific equipment

• HS Code:

  38221900

• eClass Number:

  32160405

• eClass Product Group:

  Biochemikalie (Labor, Sonstige)

• Autoclavability:

  false

• Shipping Conditions:

  Shipped on gel packs

• Storage Conditions:

  Avoid freeze/thaw cycles

• Storage Temperature:

  Store at -20 °C

• Shelf Life:

  12 months after date of delivery