AF6-RBDGST (residues 1-141) GTP-H-Ras binding cytoplasmic protein, Afadin - Ras-binding domain human, recombinant, E. coli

AF6-RBDGST (residues 1-141) GTP-H-Ras binding cytoplasmic protein, Afadin - Ras-binding domain human, recombinant, E. coli

• Description: The amino terminus of Af6 was identified as the Rasbinding site. Additionally to the Ras-binding Af6 also binds Rap. ZO-1, a protein involved in the formation of tight junctions, also binds to Af6 close to the amino terminus thereby competing with Ras binding. These data suggest a participation of Af6 in the regulation of cell-cell contacts via a Ras-modulated interaction with ZO-1. In contrast to the well characterized effectors Raf, RalGEF, and PI(3)kinase, Af6 is a protein with no enzymatic function. It seems to serve as a scaffolding component for protein complexes. This would represent a new type of Ras effector because Ras binding in this case does not lead to the activation of the enzymatic activity of the effector.
• CAS Number: 9000-83-3
• UNSPSC: 12352202
• UNSPSC Description: Proteins
• HS Code: 35079090
• Accession Number: NM_005936
• eClass Number: 34160400
• eClass Product Group: Peptide and Protein
• Purity: > 90 % (SDS-PAGE)
• Form: Liquid (Supplied in 25 mM HEPES pH 7.5, 50 mM NaCl, 1 mM EDTA and 50% glycerol)
• Autoclavability: false
• Shipping Conditions: Shipped on gel packs.
• Storage Conditions: Store at -20 °C. Avoid freeze/thaw cycles.
• Shelf Life: 12 months