SCRIPT Direct RT-qPCR GreenMaster Robust real-time RT-PCR master mix with green-fluorescent DNA stain for highly sensitive and specific amplification directly from tissue, swabs or blood

• Description:

  SCRIPT Direct RT-qPCR GreenMaster is designed for quantitative real-time analysis of target RNA directly from animal- or plant tissue, swabs and blood. The mix allows robust amplification avoiding the requirement of any prior RNA purification procedures based on a green-fluorescent DNA stain that is structurally similar to SYBR® GREEN. It allows fast and easy quantification of sample RNA over a wide dynamic range with exceptional sensitivity and precision. The mix contains all reagents required for RT-qPCR (except template and primers) in a premixed 2 x concentrated ready-to-use solution. High robustness, reliability and sensitivity of the mix are based on a genetically engineered reverse transcriptase and an antibody-blocked hot start polymerase in combination with an optimized and well-balanced buffer system. The mix ensures fast and easy preparation with a minimum of pipetting steps and is specially recommended for: Direct amplification of target RNA from various tissues samples Direct detection of viral or bacterial RNA in nasal or throat swabs Direct PCR from whole samples Point-of-Care diagnostics. Green-fluorescent DNA stain Green-fluorescent DNA stain is structurally similar to SYBR® GREEN and a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable, providing convenience during routine handling. Green-fluorescent DNA stain is not recommended for high-resolution melting curve analysis (HRM). To perform the assay, simply select the optical setting for SYBR® GREEN on the detection instrument.

• UNSPSC:

  41106300

• UNSPSC Description:

  Polymerase chain reaction PCR and reverse transcriptase polymerase chain reaction RT PCR products

• HS Code:

  35079090

• eClass Number:

  32160502

• eClass Product Group:

  PCR Reagent (Laboratory)

• Concentration:

  2x

• Form:

  Liquid

• Autoclavability:

  false

• Shipping Conditions:

  Shipped on gel packs.

• Storage Conditions:

  Store at -20 °C. Avoid freeze/thaw cycles, store dark. Stable at 4 °C for up to 4 weeks

• Shelf Life:

  12 months