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SCRIPT Direct RT-qPCR ProbesMaster Robust real-time RT-PCR master mix for highly sensitive and specific amplification directly from tissue, swabs or blood

CAT:
220-PCR-528L
Size:
10 x 1.25 mL
Price:
Ask
For price, please contact [email protected]
  • Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
  • Dry Ice Shipment: No
SCRIPT Direct RT-qPCR ProbesMaster Robust real-time RT-PCR master mix  for highly sensitive and specific amplification directly from tissue, swabs or blood - image 1

SCRIPT Direct RT-qPCR ProbesMaster Robust real-time RT-PCR master mix for highly sensitive and specific amplification directly from tissue, swabs or blood

  • Description: SCRIPT Direct RT-qPCR ProbesMaster is designed for quantitative real-time analysis of target RNA directly from animal- or plant tissue, swabs and blood. The mix allows robust amplification avoiding the requirement of any prior RNA purification procedures. The mix is recommended for use with dual-labeled fluorescent probes, e.g. TaqMan®, Molecular Beacon or Scorpion probes. It provides a powerful tool for multiplex-quantification of sample RNA in a broad dynamic range with exceptional sensitivity and precision. The mix contains all reagents required for RT-qPCR (except template, primer and labeled fluorescent probe) in a premixed 2 x concentrated ready-to-use solution. High robustness, reliability and sensitivity of the mix are based on a genetically engineered reverse transcriptase and an antibody-blocked hot start polymerase in combination with an optimized and well-balanced buffer system. The mix ensures fast and easy preparation with a minimum of pipetting steps and is specially recommended for: Direct amplification of target RNA from various tissues samples Direct detection of viral or bacterial RNA in nasal or throat swabs Direct PCR from blood samples Point-of-Care diagnostics Multiplexing Capability Real-time RT-PCR technology based on dual-labeled DNA probes provides a high sensitive and specific RT-PCR system with multiplexing capability. The simultaneous detection of multiple targets in a single tube requires a primer/probe set for each target amplification. Sequences and concentrations of primers and probes should be optimized to avoid mutual influence, secondary structures and primer-dimer formations. Amplification of each target is detected in a separate fluorescence channel.
  • UNSPSC: 41106300
  • UNSPSC Description: Polymerase chain reaction PCR and reverse transcriptase polymerase chain reaction RT PCR products
  • HS Code: 35079090
  • eClass Number: 32160502
  • eClass Product Group: PCR Reagent (Laboratory)
  • Concentration: 2x
  • Form: Liquid
  • Autoclavability: false
  • Shipping Conditions: Shipped on gel packs.
  • Storage Conditions: Store at -20 °C. Avoid freeze/thaw cycles. Stable at 4 °C for up to 4 weeks
  • Shelf Life: 12 months

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