SCRIPT High Fidelity RT-PCR Kit

CAT:

220-PCR-510S

Size:

100 Reactions x 50 µL

Price:

Ask

For price, please contact [email protected]

Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Dry Ice Shipment: No

SCRIPT High Fidelity RT-PCR Kit

Description: SCRIPT High Fidelity RT-PCR Kit is the ideal choice for applications where highly sensitive reverse transcription and high fidelity PCR at high amplification speed in single tubes are required. The enzyme mix is based on a genetically engineered reverse transcriptase with enhanced thermal stability providing increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments. Additionally, a genetically engineered proof-reading enzyme is implemented as polymerase. It provides a 50-fold higher accuracy and an increased processivity compared to Taq, resulting in 2-fold shorter elongation times. The kit contains all reagents required for RT-PCR (except template and primer) in one box to ensure fast and easy preparation with a minimum of pipetting steps. The premium quality enzyme mix and the optimized complete reaction buffer containing ultrapure dNTPs ensure superior amplification results. RT-PCR is used to amplify double-stranded DNA from single-stranded RNA templates. In the RT step the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. In the first cycle of the PCR step Taq DNA polymerase synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. During subsequent rounds of cycling the DNA polymerase exponentially amplifies this double-stranded DNA template. In one-step RT-PCR all components of RT and PCR are mixed in one tube prior to starting the reaction and thus carried out sequentially without opening the tube. This offers tremendous convenience when applied to analysis of single targets from multiple samples of RNA and minimizes the risk of contaminations.
Synonyms: One-Step RT-PCR Kit for highly precise and fast amplification
UNSPSC: 41106300
UNSPSC Description: Polymerase chain reaction PCR and reverse transcriptase polymerase chain reaction RT PCR products
HS Code: 35079090
eClass Number: 32160502
eClass Product Group: PCR-Reagenz labor
Form: Liquid
Autoclavability: false
Shipping Conditions: Shipped on gel packs
Storage Conditions: Store at -20 °C Avoid freeze/thaw cycles
Shelf Life: 12 months

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PCR-510S	SCRIPT High Fidelity RT-PCR Kit
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PCR-505l	SCRIPT Reverse Transcriptase
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