Aminoallyl-dUTP - Solution

• Description:

  Aminoallyl-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR, Nick Translation, Primer Extension, 3'-End Labeling and Reverse Transcription. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Amine-functionalized DNA/cDNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice to introduce a Biotin group (via NHS Ester of Biotin) for subsequent purification tasks to introduce fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging

• UNSPSC:

  12352208

• UNSPSC Description:

  Nucleic acids

• HS Code:

  29349990

• eClass Number:

  34160990

• eClass Product Group:

  Nucleinsäure (nicht klassifiziert)

• Applications:

  Incorporation into DNA/cDNA by PCR with Taq polymerase[1], in-house data Nick Translation with DNAse I/ DNA Polymerase I[2] Primer Extension with Klenow exo- [3] 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT)[4] Reverse Transcription with MMLV Reverse Transcriptase[2]

• Concentration:

  (100 mM)

• Purity:

  ≥ 95 % (HPLC)

• Form:

  Solution in water

• pH:

  7.5 ±0.5

• Color:

  Colorless to slightly yellow

• Autoclavability:

  false

• Smiles:

  NC/C=C/c1cn (c (=O) [nH]c1=O) [C@H]1C[C@@H] ([C@H] (O1) COP (=O) (O) OP (=O) (O) OP (=O) (O) O) O

• Shipping Conditions:

  Shipped on gel packs

• Storage Conditions:

  Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.

• Storage Temperature:

  Store at -20 °C

• Notes:

  Short term exposure (up to 1 week cumulative) to ambient temperature possible.

• Shelf Life:

  12 months after date of delivery

• CAS Number:

  116840-18-7