Aminoallyl-dUTP - Solution

• Description :

  Aminoallyl-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR, Nick Translation, Primer Extension, 3'-End Labeling and Reverse Transcription. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Amine-functionalized DNA/cDNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice to introduce a Biotin group (via NHS Ester of Biotin) for subsequent purification tasks to introduce fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging

• UNSPSC :

  12352208

• UNSPSC Description :

  Nucleic acids

• HS Code :

  29349990

• eClass Number :

  34160990

• eClass Product Group :

  Nucleinsäure (nicht klassifiziert)

• Applications :

  Incorporation into DNA/cDNA by PCR with Taq polymerase[1], in-house data Nick Translation with DNAse I/ DNA Polymerase I[2] Primer Extension with Klenow exo- [3] 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT)[4] Reverse Transcription with MMLV Reverse Transcriptase[2]

• Concentration :

  (100 mM)

• Purity :

  ≥ 95 % (HPLC)

• Form :

  Solution in water

• pH :

  7.5 ±0.5

• Color :

  Colorless to slightly yellow

• Autoclavability :

  false

• Smiles :

  NC/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O

• Shipping Conditions :

  Shipped on gel packs

• Storage Conditions :

  Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.

• Storage Temperature :

  Store at -20 °C

• Notes :

  Short term exposure (up to 1 week cumulative) to ambient temperature possible.

• Shelf Life :

  12 months after date of delivery

• CAS Number :

  116840-18-7