Aminoallyl-dUTP - Solution

CAT: 0220-NU-803LSize: 5x 10 µLDry Ice: NoHazardous: No
CAT#:0220-NU-803LSize:5x 10 µL
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Aminoallyl-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR, Nick Translation, Primer Extension, 3'-End Labeling and Reverse Transcription. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Amine-functionalized DNA/cDNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice to introduce a Biotin group (via NHS Ester of Biotin) for subsequent purification tasks to introduce fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging
CAS Number
116840-18-7
UNSPSC
12352208
UNSPSC Description
Nucleic acids
HS Code
29349990
eClass Number
34160990
eClass Product Group
Nucleinsäure (nicht klassifiziert)
Applications
Incorporation into DNA/cDNA by PCR with Taq polymerase[1], in-house data Nick Translation with DNAse I/ DNA Polymerase I[2] Primer Extension with Klenow exo- [3] 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT)[4] Reverse Transcription with MMLV Reverse Transcriptase[2]
Concentration
(100 mM)
Purity
≥ 95 % (HPLC)
Form
Solution in water
pH
7.5 ±0.5
Color
Colorless to slightly yellow
Autoclavability
—
Smiles
NC/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O
Shipping Conditions
Shipped on gel packs
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Storage Temperature
Store at -20 °C
Notes
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life
12 months after date of delivery
Product Datasheet
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Product MSDS
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