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Fluorescein-12-dUTP

CAT: 0220-NU-803-FAMX-LSize: 5x 10 μLDry Ice: NoHazardous: No
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CAT#:0220-NU-803-FAMX-LSize:5x 10 μL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Fluorescein-12-dUTP is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Fluorescein-12-dUTP/dTTP ratio for PCR and Nick Translation: 30-50% Fluorescein-12-dUTP/ 70-50% dTTP
Product Name Alternative
Fluorescein-X- (5-aminoallyl) -dUTP
UNSPSC
12352208
UNSPSC Description
Nucleic acids
HS Code
29349990
eClass Number
34160990
eClass Product Group
Nucleinsäure (nicht klassifiziert)
Applications
Incorporation into DNA/cDNA by - PCR with Taq polymerase in-house data, [1] - Nick Translation with DNAse I/ DNA Polymerase I in-house data
Concentration
(1 mM)
Purity
≥ 95 % (HPLC)
Form
Solution in water
pH
7.5 ±0.5
Color
Yellow-orange
Autoclavability
—
Smiles
O=c1ccc2c(c1)oc1cc(O)ccc1c2c1c(ccc(c1)C(=O)NCCCCCC(=O)NC/C=C/c1cn(c(=O)[nH]c1=O)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1)C(=O)O.O=c1c(cn(c(=O)[nH]1)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1)/C=C/CNC(=O)CCCCCNC(=O)c1cc(c(c2c3ccc(O)cc3oc3cc(=O)ccc23)cc1)C(=O)O
Shipping Conditions
Shipped on gel packs
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Storage Temperature
Store at -20 °C
Notes
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life
12 months after date of delivery
Product Datasheet
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Product MSDS
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