Aminoallyl-dUTP-ATTO-550

• Description:

  Aminoallyl-dUTP-ATTO550 is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-ATTO550/dTTP ratio Nick Translation: 30-50% Aminoallyl-dUTP-ATTO550/ 50% dTTP

• UNSPSC:

  12352208

• UNSPSC Description:

  Nucleic acids

• HS Code:

  29349990

• eClass Number:

  34160990

• eClass Product Group:

  Nucleinsäure (nicht klassifiziert)

• Applications:

  Incorporation into DNA/cDNA by - Nick Translation with DNAse I/ DNA Polymerase I in-house data

• Concentration:

  (1 mM)

• Purity:

  ≥ 95 % (HPLC)

• Form:

  Filtered solution (30 kDa) in 10 mM Tris-HCl

• pH:

  7.5 ±0.5

• Color:

  Red-violet

• Autoclavability:

  false

• Smiles:

  [C@@H]1 (C[C@@H] (O[C@@H]1COP (=O) (OP (=O) (OP (=O) ([O-]) O) O) O) n1c (=O) [nH]c (=O) c (c1) /C=C/CNC (=O) CCCN (C (=O) c1c (C2=c3cc4C (CC ([NH+]=c4cc3Oc3cc4NC (CC (c4cc23) C) (C) C) (C) C) C) cccc1) C) O

• Shipping Conditions:

  Shipped on gel packs

• Storage Conditions:

  Store at -20 °C Short term exposure up to 1 week cumulative to ambient temperature possible.

• Storage Temperature:

  Store at -20 °C

• Notes:

  Short term exposure (up to 1 week cumulative) to ambient temperature possible.

• Shelf Life:

  12 months after date of delivery