PCp-Biotin

• Description:

  pCp-Biotin is recommended for 3'-End Labeling of RNA/miRNA with T4 RNA Ligase that conjugates a single Biotin molecule to the 3'-OH group of the RNA/miRNA template. The resulting Biotin-labeled RNA/miRNA probes are ideally suited for hybridization experiments such as microarray-based expression profiling or electro mobility shift assays (EMSA). Detection of the Biotin moiety can be performed using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. Alternatively, Biotin-labeled RNA/miRNA probes can also be purified with Streptavidin-conjugated agarose/magnetic beads.

• UNSPSC:

  12352208

• UNSPSC Description:

  Nucleic acids

• HS Code:

  29349990

• eClass Number:

  34160990

• eClass Product Group:

  Nucleinsäure (nicht klassifiziert)

• Applications:

  Incorporation into RNA/miRNA by 3'-End Labeling with T4 RNA Ligase

• Concentration:

  (1 mM)

• Purity:

  ≥ 95 % (HPLC)

• Form:

  Solution in 10 mM Tris-HCl

• pH:

  7.5 ±0.5

• Color:

  Colorless to slightly yellow

• Autoclavability:

  false

• Smiles:

  Nc1nc (=O) n (cc1) [C@@H]1O[C@H] (COP (=O) (O) O) [C@H] ([C@H]1O) OP (=O) (OCCCCCCNC (=O) CCCC[C@@H]1SC[C@H]2[C@@H]1NC (=O) N2) O

• Shipping Conditions:

  Shipped on gel packs

• Storage Conditions:

  Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.

• Storage Temperature:

  Store at -20 °C

• Notes:

  Short term exposure (up to 1 week cumulative) to ambient temperature possible.

• Shelf Life:

  12 months after date of delivery