PCp-Biotin

• Description :

  pCp-Biotin is recommended for 3'-End Labeling of RNA/miRNA with T4 RNA Ligase that conjugates a single Biotin molecule to the 3'-OH group of the RNA/miRNA template. The resulting Biotin-labeled RNA/miRNA probes are ideally suited for hybridization experiments such as microarray-based expression profiling or electro mobility shift assays (EMSA). Detection of the Biotin moiety can be performed using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. Alternatively, Biotin-labeled RNA/miRNA probes can also be purified with Streptavidin-conjugated agarose/magnetic beads.

• UNSPSC :

  12352208

• UNSPSC Description :

  Nucleic acids

• HS Code :

  29349990

• eClass Number :

  34160990

• eClass Product Group :

  Nucleinsäure (nicht klassifiziert)

• Applications :

  Incorporation into RNA/miRNA by 3'-End Labeling with T4 RNA Ligase

• Concentration :

  (1 mM)

• Purity :

  ≥ 95 % (HPLC)

• Form :

  Solution in 10 mM Tris-HCl

• pH :

  7.5 ±0.5

• Color :

  Colorless to slightly yellow

• Autoclavability :

  false

• Smiles :

  Nc1nc(=O)n(cc1)[C@@H]1O[C@H](COP(=O)(O)O)[C@H]([C@H]1O)OP(=O)(OCCCCCCNC(=O)CCCC[C@@H]1SC[C@H]2[C@@H]1NC(=O)N2)O

• Shipping Conditions :

  Shipped on gel packs

• Storage Conditions :

  Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.

• Storage Temperature :

  Store at -20 °C

• Notes :

  Short term exposure (up to 1 week cumulative) to ambient temperature possible.

• Shelf Life :

  12 months after date of delivery