5-Azido-C3-UTP

5-Azido-C3-UTP

• UNSPSC: 12352208
• UNSPSC Description: Nucleic acids
• HS Code: 29349990
• eClass Number: 34160990
• eClass Product Group: Nucleic Acid (Unclassified)
• Applications: Incorporation into RNA by T7 RNA polymerase-mediated in vitro transcription (up to 1400 bp tested). For a comprehensive protocol please refer to PP-501 or PP-501-Cy3/Cy5. 3'-Azide-Labeling of T7 promotor-containing oligonucleotides[1,2,3] The resulting azide-functionalized RNA can subsequently be processed via Cu(I)-free (azide-DBCO) or Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choice - to introduce a Biotin group for subsequent purification tasks (via DBCO-functionalized Biotin or Alkynes of Biotin, respectively) - to introduce fluorescent group for subsequent microscopic imaging (DBCO-functionalized fluorescent dyes or Alkynes of fluorescent dyes, respectively) - to crosslink the RNA to azide- or alkyne functionalized biomolecules e.g.proteins
• Concentration: 10 mM - 11 mM
• Purity: ≥ 95 % (HPLC)
• Form: Solution in water
• pH: 7.5 ±0.5
• Color: Colorless to slightly yellow
• Autoclavability: false
• Smiles: C(CCc1cn(c(=O)[nH]c1=O)[C@H]1[C@@H]([C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N=[N+]=[N-]
• Shipping Conditions: Shipped on gel packs.
• Storage Conditions: Store at -20 °C. Avoid freeze/thaw cycles. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
• Shelf Life: 12 months after date of delivery