Biotin-11-dATP

• Description :

  Biotin-11-dATP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the 7-Deaza position of adenine.

• UNSPSC :

  12352208

• UNSPSC Description :

  Nucleic acids

• HS Code :

  29349990

• eClass Number :

  34160990

• eClass Product Group :

  Nucleinsäure (nicht klassifiziert)

• Applications :

  Incorporation into DNA/cDNA by - PCR with Taq polymerase [1] & in-house data - Nick Translation with DNAse I/ DNA Polymerase I [2] - Primer Extension with Klenow exo- [3,4] - Reverse Transcription with MMLV Reverse Transcriptase [5]

• Concentration :

  (1 mM)

• Purity :

  ≥ 95 % (HPLC)

• Form :

  Filtered solution (30 kDa) in 10 mM Tris-HCl

• pH :

  7.5 ±0.5

• Color :

  Colorless to slightly yellow

• Autoclavability :

  false

• Smiles :

  N1C(=O)N[C@@H]2[C@H]1[C@H](CCCCC(=O)NCCCCCC(=O)NCC#Cc1cn(c3ncnc(N)c13)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1)SC2

• Shipping Conditions :

  Shipped on gel packs

• Storage Conditions :

  Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.

• Storage Temperature :

  Store at -20 °C

• Notes :

  Short term exposure (up to 1 week cumulative) to ambient temperature possible.

• Shelf Life :

  12 months after date of delivery