Turbo Nuclease also known as Benzonase® Serratia marcescens, recombinant, E. coli

Turbo Nuclease also known as Benzonase® Serratia marcescens, recombinant, E. coli

• Description: Turbo Nuclease is a broad-spectrum endonuclease that cleaves both DNA and RNA molecules independently of being single- or double-stranded, circular, linear or supercoiled. The enzyme is highly stable and active in a broad range of pH and temperature, making it ideal for a variety of downstream processes that require the degradation of DNA/RNA in a simple, efficient and specific manner. Endonuclease from Serratia macescens recombinant expressed and purified from E. coli Catalytic activity from pH 6 to 10 (optimal around 8.8) and temperature 0 to 44 °C High catalytic efficiency (34-fold greater than DNase I) Activity requires the presence of Mg2+ (optimum 2 mM)
• CAS Number: 9000-83-3
• UNSPSC: 12352204
• UNSPSC Description: Enzymes
• HS Code: 35079090
• eClass Number: 34160500
• eClass Product Group: Enzyme and Coenzyme
• Applications: Turbo Nuclease is very effective in degrading nucleic acid from protein samples and it is used in a variety of application where complete hydrolysis of DNA/RNA is required: Reduction of viscosity from cell lysates Prevention of cell clumping Elimination of unspecific protein-DNA complexes prior 2D- or native gel electrophoresis Removal of nucleic acids from large-scale protein preparations
• Concentration: 250 units/μL
• Form: Liquid (Supplied in 20 mM Tris-HCl pH 8.0, 20 mM NaCl, 2 mM MgCl2, 1 mM DTT and 50 % [v/v] glycerol)
• Autoclavability: false
• Shipping Conditions: Shipped on gel packs.
• Storage Conditions: Store at -20 °C. Avoid freeze/thaw cycles.
• Shelf Life: 12 months