Klenow Fragment Large Fragment of DNA Polymerase I recombinant, E. coli

• Description:

  Klenow Fragment is the large fragment of DNA Polymerase I that retains its 5'→3' polymerase, 3'→5' exonuclease and strand displacement activities. The enzyme lacks the 5'→3' exonuclease activity of intact DNA polymerase I. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini.

• UNSPSC:

  12352204

• UNSPSC Description:

  Enzymes

• HS Code:

  35079090

• eClass Number:

  34160500

• eClass Product Group:

  Enzyme and Coenzyme

• Applications:

  Fill-in of 5' overhangs to form blunt ends Removal of 3' overhangs to form blunt ends

• Concentration:

  5 units/μL

• Form:

  Liquid (Supplied in 100 mM KPO4 pH 6.5, 1 mM DTT and 50 % [v/v] glycerol)

• Autoclavability:

  false

• Shipping Conditions:

  Shipped on gel packs.

• Storage Conditions:

  Store at -20 °C. Avoid freeze/thaw cycles.

• Shelf Life:

  12 months

• CAS Number:

  9000-83-3