5-Ethynyl-2'-deoxycytidine (5-EdC)

5-Ethynyl-2'-deoxycytidine (5-EdC)

• Description: Ethynyl-labeled deoxycytidine (5-EdC) can be used as a replacement for BrdU (5-Bromo-2’-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle. 5-EdC is cell-permeabel and incorporates into replicating DNA instead of its natural analog thymidine. The resulting ethynyl-functionalized DNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (Azides of Biotin) for subsequent purification tasks or a fluorescent group (Azides of fluorescent dyes) for subsequent microscopic imaging [1].
• CAS Number: 9000-83-3
• UNSPSC: 12352200
• UNSPSC Description: Biochemicals
• HS Code: 29349990
• eClass Number: 32160405
• eClass Product Group: Biochemical (Laboratory, Miscellaneous)
• Applications: DNA synthesis monitoring[1]
• Purity: ≥ 99 % (HPLC)
• Form: Solid
• Color: Off-white
• Autoclavability: false
• Solubility: DMSO
• Smiles: n1(cc(c(nc1=O)N)C#C)[C@H]1C[C@@H]([C@H](O1)CO)O
• Shipping Conditions: Shipped at ambient temperature.
• Storage Conditions: Store at -20 °C. Store dry and under inert gas. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
• Shelf Life: 12 months after date of delivery
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