5-Azidomethyl-2'-deoxyuridine (5-AmdU)

5-Azidomethyl-2'-deoxyuridine (5-AmdU)

• Description: 5-AmdU (5-Azidomethyl-2'-deoxyuridine) can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle. 5-AmdU is cell permeable and incorporates into peplicating DNA instead of its natural analog thymidine. The resulting azide-functionalized DNA can subsequently be detected via Cu(I)-catalyzed (CuAAC) or Cu(I)-free Click Chemistry that offers the choice to introduce a Biotin group for subsequent purification tasks (via Alkynes of Biotin or DBCO-functionalized Biotin, respectivly) introduce a fluorescent group for subsequent microscopic imaging (via Alkynes of fluorescent dyes or DBCO-functionalized fluorescent dyes, respectivly).
• UNSPSC: 12352208
• UNSPSC Description: Nucleic acids
• HS Code: 29349990
• eClass Number: 34160990
• eClass Product Group: Nucleic Acid (Unclassified)
• Applications: DNA synthesis monitoring[1]
• Purity: ≥ 90 % (HPLC)
• Form: Solid
• Color: White to off-White
• Autoclavability: false
• Solubility: DMSO, methanol
• Smiles: O=c1c(cn(c(=O)[nH]1)[C@@H]1O[C@H](CO)[C@@H](O)C1)CN=[N+]=[N-]
• Shipping Conditions: Shipped at ambient temperature.
• Storage Conditions: Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
• Shelf Life: 12 months after date of delivery