5-DBCO-PEG4-dUTP

5-DBCO-PEG4-dUTP

• Description: 5-DBCO-PEG4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting DBCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins
• UNSPSC: 12352200
• UNSPSC Description: Biochemicals
• HS Code: 29349990
• eClass Number: 32160405
• eClass Product Group: Biochemical (Laboratory, Miscellaneous)
• Applications: Incorporation into DNA/cDNA by PCR with Taq polymerase in-house data
• Concentration: 10 mM - 11 mM
• Purity: ≥ 95 % (HPLC)
• Form: Solution in 100 mM Tris-HCl
• pH: 7.5 ±0.5
• Color: Colorless to slightly yellow
• Autoclavability: false
• Smiles: c1(=O)[nH]c(=O)n(cc1/C=C/CNC(=O)CCOCCOCCOCCOCCNC(=O)CCC(=O)N1Cc2c(C#Cc3c1cccc3)cccc2)[C@H]1C[C@H](O)[C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O
• Shipping Conditions: Shipped on gel packs.
• Storage Conditions: Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
• Shelf Life: 12 months after date of delivery