5-DBCO-PEG4-dUTP

• Description:

  5-DBCO-PEG4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting DBCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins

• UNSPSC:

  12352200

• UNSPSC Description:

  Biochemicals

• HS Code:

  29349990

• eClass Number:

  32160405

• eClass Product Group:

  Biochemikalie (Labor, Sonstige)

• Applications:

  Incorporation into DNA/cDNA by PCR with Taq polymerase in-house data

• Concentration:

  (10 mM)

• Purity:

  ≥ 95 % (HPLC)

• Form:

  Solution in 100 mM Tris-HCl

• pH:

  7.5 ±0.5

• Color:

  Colorless to slightly yellow

• Autoclavability:

  false

• Smiles:

  C1 (=O) [nH]c (=O) n (cc1/C=C/CNC (=O) CCOCCOCCOCCOCCNC (=O) CCC (=O) N1Cc2c (C#Cc3c1cccc3) cccc2) [C@H]1C[C@H] (O) [C@H] (O1) COP (=O) (O) OP (=O) (O) OP (=O) (O) O

• Shipping Conditions:

  Shipped on gel packs

• Storage Conditions:

  Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.

• Storage Temperature:

  Store at -20 °C

• Notes:

  Short term exposure (up to 1 week cumulative) to ambient temperature possible.

• Shelf Life:

  12 months after date of delivery