anti-Pam-Igloo-L anti Periplaneta Americana Igloo L 1-75 rabbit, polyclonal

anti-Pam-Igloo-L anti Periplaneta Americana Igloo L 1-75 rabbit, polyclonal

• Description: Axogenesis and synaptic signal transmission in the vertebrate nervous system are based on the abundant expression of the growth-associated protein GAP-43. In non-vertebrates a GAP-43 related gene (igloo, invertebrate GAP-43 like) was found in Drosophila and encodes two proteins, IGLOO-L and IGLOO-S, with sequence homology and similar biochemical activity to GAP-43. We have cloned a GAP-43-like gene in a holometabolic insect, the American cockroach, Periplaneta americana. As for Drosophila, we found two putative pigl mRNAs, pigl-L and pigl-S, in the cockroach (Fig. 1). Immunocytochemical investigations with a antiserum to Pam-Igl L and Pam-Igl S provide a function for Pam-Igloo L in establishment or maintenance of synaptic contacts and can help to understand the ongoing expression of GAP-43 in the mature nervous system (Hänold,2004).
• Specifications: The specificity against P-IGL-L, but not P-IGL-S, was demonstrated by transfected CHO cells, where the antibody selectively recognizes IGL-L. This P-IGL-L-like immunoreactivity (P-IGL-L-li) was colocalized with the fluorescence signal from the IGL-L-EGFP fusion protein. Furthermore, the antiserum was tested on neuronal tissue sections of the American cockroach: P-IGL-L-li could be blocked by preincubation of the antibody with recombinant P-IGL-L protein. There was also no immunostaining after replacement of antiserum with preimmune serum (Fig 2). The specificity of the antiserum against Periplaneta americana IGL-L was investigated by immunostaining of brain sections from different cockroach species.
• CAS Number: 9007-83-4
• UNSPSC: 12352203
• UNSPSC Description: Antibodies
• HS Code: 30021500
• eClass Number: 32160702
• eClass Product Group: Antigen, Antibody (Laboratory)
• Applications: Anti-Pam-Igloo-L can be used for ELISA and Immunocytochemistry.
• Form: Liquid (Supplied as serum, preserved in glycerol)
• Autoclavability: false
• Shipping Conditions: Shipped on gel packs.
• Storage Conditions: Store at -20 °C. Avoid freeze/thaw cycles.
• Shelf Life: 12 months