Alkaline Phosphatase Conjugated Affinity Purified anti-Donkey IgG [H&L] [Goat]

• Reactivity:

  Donkey

• Immunogen:

  Donkey IgG whole molecule

• Type:

  Antibodies-Secondary

• Applications:

  Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase- based enzymatic assays requiring lot-to-lot consistency.

• Concentration:

  0.59 mg/ml (by UV absorbance at 280 nm)

• Purity:

  This product was prepared from monospecific antiserum by immunoaffinity chromatography using Donkey IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Donkey IgG, and Donkey Serum.

• Buffer:

  0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0

• Shipping Conditions:

  Ice Pack

• Storage Conditions:

  Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.

• Applications Notes:

  This product has been assayed against 1.0 µg of Donkey IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:16,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.

• CAS Number:

  9007-83-4