Anti-Interleukin-6 (IL-6) Mouse

• Description:

  Rabbit polyclonal to IL6

• Reactivity:

  Mouse

• Immunogen:

  highly pure (>98%) recombinant mouse IL-6

• Clonality:

  Polyclonal

• Type:

  Antibodies-Polyclonal

• Applications:

  WB,ELISA,Other

• Purity:

  Affinity purified

• Buffer:

  PBS buffer, pH 7.4. Reconstitute in sterile water at a concentration of >0.2 mg/ml.

• Shipping Conditions:

  Ice Pack

• Storage Conditions:

  This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

• Specificity:

  mIL-6100% reactivity

• Applications Notes:

  Neutralization:To yield one-half maximal inhibition [ND₅₀] of the biological activity of mIL-6 (0.1 ng/ml), a concentration of 0.012 - 0.014 µg/ml of this antibody is required. ELISA:To detect mIL-6 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-6. Western Blot:To detect mIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions

• CAS Number:

  9007-83-4