Anti-Neurofilament

• Description:

  Neurofilaments comprise the exoskeleton and functionally maintain the quality of neurons. They are composed of light, medium, and heavy chains. Encoded by the NEFH gene, the heavy neurofilament protein is commonly used as a biomarker of neuronal damage. Mutations in the NEFH gene have also been associated with susceptibility to amyotrophic lateral sclerosis (ALS). The Anti-Neurofilament Antibody robustly recognizes polyphosphorylated epitopes on neurofilaments and to a much lesser extent, on other molecules such as histones and rhodopsin. The antibody also reacts with neurofibrillary tangles and plaques and cross-reacts with neurofilament medium. Thus, the Anti-Neurofilament Antibody can be used as a cell marker for neurons.

• Reactivity:

  Human,Mouse,Rat,Bovine,Avian

• Immunogen:

  Semi-purified neurofilaments from homogenate of rat brain.

• Clonality:

  Monoclonal

• Type:

  Antibodies-Monoclonal

• Applications:

  WB,IHC

• Concentration:

  100µg/100µl

• Purity:

  The antibody was affinity-purified from hybridoma supernatant by affinity-chromatography.

• Buffer:

  PBS, pH 7.4 with 0.05% sodium azide

• Shipping Conditions:

  Ice Pack

• Storage Conditions:

  This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

• Fragment:

  IgG1

• Specificity:

  Anti-Neurofilament Antibody recognizes Neurofilament-Heavy (NF-H) and stains sharply defined cytoplasmic filaments in neuronal cell bodies particularly axons.

• Applications Notes:

  WB and IHC: 1:1000~1:10000 Optimal dilutions should be calculated by the end user based on their specific application.

• CAS Number:

  9007-83-4