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Anti-Human IL-10

CAT: 0171-Y050209Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0171-Y050209Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
9007-83-4
Reactivity
Human
Immunogen
The whole rabbit serum was prepared by repeated immunizations with recombinant human IL-10 produced in E.coli.
Clonality
Polyclonal
Type
Antibodies-Polyclonal
Applications
WB,IHC,ELISA,IP,Other
Concentration
1ug/ul
Purity
Purified
Buffer
PBS, pH 7.4 with 0.05% sodium azide.
Shipping Conditions
Ice Pack
Storage Conditions
This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Fragment
IgG
Specificity
This antiserum has been heated to 56°C for 30 minutes. The antiserum detects recombinant and native IL-10 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL- 10 production from fibroblasts). In Western blot analysis of natural cell products_recover or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule. The antiserum is also useful for neutralization of human of IL-10 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. The antibody will not neutralize the biological activity of murine IL-10.
Applications Notes
This antiserum has been tested for use in neutralizations, ELISA, immunohistochemistry, radioimmunoassay, immunoprecipitation, immunohistochemistry and immunoblotting. The antibody recognizes hrIL-10. Reactivity in other immunoassays is unknown.

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