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Anti-Cas9

CAT: 0171-Y300079Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0171-Y300079Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Anti-Cas9 Antibody is an antibody against Cas9, an essential RNA-guided DNA endonuclease observed originally in Streptococcus pyogene among other bacteria and archaea and a component of an adaptive microbial immune system that provides acquired immunity against foreign viruses and plasmids. Cas9 endonuclease is used to cleave double-stranded DNA directed by a short engineered guide-RNA (gRNA), leading to site-directed double strand breaks (DSB) in DNA submstrate. Thus, the CRISPR Cas9 system (clustered regularly interspaced short palindromic repeat CRISPR-associated protein 9) is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement.

Predicted size of 160kDa.
CAS Number
9007-83-4
Reactivity
Other
Immunogen
Recombinant fragment corresponding to Cas9 endonuclease from Streptococcus pyogenes.
Clonality
Monoclonal
Type
Antibodies-Monoclonal
Applications
WB,ELISA
Concentration
100 μg / 100 μl
Purity
Affinity purified from ascites fluid.
Buffer
PBS, pH 7.4 with 0.02% sodium azide.
Shipping Conditions
Ice Pack
Storage Conditions
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Fragment
IgG1
Applications Notes

Working dilution must be determined by the user.

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