Anti-Cas9

• Description:

  Anti-Cas9 Antibody is an antibody against Cas9, an essential RNA-guided DNA endonuclease observed originally in Streptococcus pyogene among other bacteria and archaea and a component of an adaptive microbial immune system that provides acquired immunity against foreign viruses and plasmids. Cas9 endonuclease is used to cleave double-stranded DNA directed by a short engineered guide-RNA (gRNA), leading to site-directed double strand breaks (DSB) in DNA submstrate. Thus, the CRISPR Cas9 system (clustered regularly interspaced short palindromic repeat CRISPR-associated protein 9) is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement.
  
  Predicted size of 160kDa.

• Reactivity:

  Other

• Immunogen:

  Recombinant fragment corresponding to Cas9 endonuclease from Streptococcus pyogenes.

• Clonality:

  Monoclonal

• Type:

  Antibodies-Monoclonal

• Applications:

  WB,ELISA

• Concentration:

  100 μg / 100 μl

• Purity:

  Affinity purified from ascites fluid.

• Buffer:

  PBS, pH 7.4 with 0.02% sodium azide.

• Shipping Conditions:

  Ice Pack

• Storage Conditions:

  This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

• Fragment:

  IgG1

• Applications Notes:

  Working dilution must be determined by the user.

• CAS Number:

  9007-83-4