Anti-PTEN (Phospho-Ser370)

• Description:

  This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq, Jul 2008]

• Reactivity:

  Human,Mouse,Rat

• Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PTEN around the phosphorylation site of serine 370 (D-V-SP-D-N).

• Clonality:

  Polyclonal

• Type:

  Antibodies-Polyclonal

• Applications:

  WB,IHC

• Concentration:

  1ug/ul

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Buffer:

  phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Shipping Conditions:

  Ice Pack

• Storage Conditions:

  This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

• Specificity:

  PTEN (phospho-Ser370) antibody detects endogenous levels of PTEN only when phosphorylated at serine 370.

• Applications Notes:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• CAS Number:

  9007-83-4