Anti-human IgE

CAT: 0171-Y059504Size: 100 µgDry Ice: NoHazardous: No
CAT#:0171-Y059504Size:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.05-0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
CAS Number
9007-83-4
Reactivity
Human
Immunogen
Myelomatous human serum
Clonality
Monoclonal
Type
Antibodies-Monoclonal
Applications
WB,ELISA
Concentration
1ug/ul
Purity
MAbs 4F4, 5D4, E411, BE5, 4G7 and 4H10 by chromatography on protein A Sepharose MAb XTE4 by ion exchange chromatography
Buffer
PBS, pH 7.4 with 0.05% sodium azide.
Shipping Conditions
Ice Pack
Storage Conditions
This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Fragment
IgG for MAbs BE5, 4G7 and 4H10 IgG1 for MAb XTE4 IgG2a for MAbs 4F4, 5D4 and E411
Specificity
MAb E411 class specific for human IgE, Fc-region specific MAb 4F4 - IgE Cε3-domain specific MAb 5D4 - IgE Cε2-domain specific MAb XTE4 - epsilon chain specific Specific antibody titer is 1/1 000 000 by indirect ELISA.
Applications Notes
Detection of total IgE level and IgE antibodies in ELISA. Recommended pairs (coating - conjugate): 5D4 – 4F4, 4G7– BE5. MAb E411 can be used for coating. MAb 4F4 can be used for IgE purification by affinity chromatography. MAb XTE4 can be used in Western blotting.

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