Anti-CD44

• Description:

  CD44 is a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.

• Reactivity:

  Human,Other

• Immunogen:

  COS-7 cells (African Green Monkey).

• Clonality:

  Monoclonal

• Type:

  Antibodies-Monoclonal

• Applications:

  WB,IP,Flow cyt

• Concentration:

  1ug/ul

• Purity:

  Affinity purified

• Buffer:

  PBS, pH 7.4 with 0.05% sodium azide.

• Shipping Conditions:

  Ice Pack

• Storage Conditions:

  This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

• Fragment:

  IgG1

• Specificity:

  The antibody reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1)

• Applications Notes:

  Western Blotting recommended dilution: 1:500 Positive control: Kg-1a human acute leukemia cell lysate JURKAT human leukemia T-cell lysate Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. 60 min on vertical incubator Application note: Non-reducing conditions. SDS-PAGE (6% separating gel).

• CAS Number:

  9007-83-4