Aminoallyl-dUTP-XX-AZDye647 structural analog to Alexa Fluor® 647

Aminoallyl-dUTP-XX-AZDye647 structural analog to Alexa Fluor® 647

• Description: Aminoallyl-dUTP-XX-AZDye647 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. AZDye 647 (structural analog to Alexa Fluor 647®) is a hydrophilic dye with excellent photostability compared to fluorescein.
• UNSPSC: 12352208
• UNSPSC Description: Nucleic acids
• HS Code: 29349990
• eClass Number: 34160990
• eClass Product Group: Nucleic Acid (Unclassified)
• Applications: Incorporation into DNA/cDNA by - PCR with Taq polymerase in-house data - Nick Translation with DNAse I/ DNA Polymerase I in-house data
• Concentration: 1.0 mM - 1.1 mM
• Purity: ≥ 95 % (HPLC)
• Form: Filtered solution (30 kDa) in 10 mM Tris-HCl
• pH: 7.5 ±0.5
• Color: Blue
• Autoclavability: false
• Smiles: P(=O)(OP(=O)(O)O)(O)OP(=O)(O)OC[C@H]1O[C@H](C[C@@H]1O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCCCCNC(=O)CCCCCNC(=O)CCCCC[N+]1=C(C(C)(c2cc(ccc12)S(=O)(=O)O)CCCCS(=O)(=O)[O-])/C=C/C=C/C=C\1/N(c2ccc(cc2C1(C)C)S(=O)(=O)O)CCCS(=O)(=O)O
• Shipping Conditions: Shipped on gel packs.
• Storage Conditions: Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
• Shelf Life: 12 months after date of delivery