Anti-TIA1 (T-Cell-Restricted Intracellular Antigen-1) (TIA1/1313), CF640R conjugate

• Description:

  TIA-1 (T-cell intracytoplasmic antigen) is a cytoplasmic granule-associated protein expressed in lymphocytes processing cytolytic potential. TIA-1 is a member of an RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15 kDa protein thought to be derived from the carboxyl terminus of the 40 kDa product by proteolytic processing. TIA1 antibody labels cytotoxic T cells and natural killer cells (NK cells). It is also expressed in T-cell lymphoma, large granular lymphocyte (LGL) leukemia and hairy cell leukemia. TIA1 expression in T-cell malignancies may help in differentiating LGL leukemia (high expression) from T-cell lymphocytosis and other T-cell diseases (low expression). TIA1 may also be used to label tumor-infiltrating lymphocytes in the study of immune response to malignancies.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Cytotoxic granule associated RNA binding protein 1; mTIA-1; Nucleolysin TIA1 isoform p40; p40-TIA-1; RNA binding protein TIA1; T-cell-restricted intracellular antigen-1 (TIA1); TIA-1; TIA1; TIA1 cytotoxic granule associated RNA binding protein; TIAL1; TIAR; WDM

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  TIA1

• Gene ID:

  7072

• NCBI Gene ID:

  413123

• UniProt:

  P31483

• Cellular Locus:

  Cytoplasmic

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant human TIA1 fragment of 102 amino acid residues (aa279-380) (exact sequence is proprietary)

• Target Antigen:

  TIA1

• Clonality:

  Monoclonal

• Isotype:

  IgG2b

• Clone:

  TIA1/1313

• Conjugation:

  CF640R

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  IF (verified) | WB (verified)

• Validated Applications:

  IF, WB

• Field of Research:

  Cancer, Immunology

• Positive Control:

  HepG2, PC3 and HeLa cells. Tonsil.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  40 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Flow cytometry: 0.5-1 ug/million cells in 0.1mL|Western blotting 1-2 ug/mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4