Recombinant Rat IGF1

• Description:

  Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I is synthesized as two precursor isoforms with N- and alternate C- terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I is identical between isoforms and is generated by proteolytic removal of the N- and C- terminal regions. Mature human IGF-I shares 94% and 96% aa sequence identity with mouse and rat IGF-I, respectively (2), and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF-II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects.

• Gene Name:

  IGF1

• Gene Aliases:

  Somatomedin, Insulin-like growth factor I, IGF-I, Igf1
  Recombinant Rat Insulin-Like Growth Factor (IGF1)

• Gene ID:

  24482

• Accession Number:

  P08025

• Organism:

  Rat

• Source:

  E. coli

• Concentration:

  <1.0 EU/μg of recombinant protein as determined by the LAL method.

• Purity:

  >95% as determined by SDS-PAGE

• Solubility:

  A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

• Function:

  The ED(50) was determined by a cell proliferation assay using FDC-P1 cells is ≤ 1.0 ng/mL, corresponding to a specific activity of ≥ 1.0 x 10^6 units/mg.

• Molecular Weight:

  8

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

• Appearance:

  Lyophilized Powder

• Formulation:

  Recombinant Rat IGF1 was lyophilized from a 0.2 μm filtered PBS solution, pH 7.4.

• CAS Number:

  9000-83-3