Recombinant Rat EGF

CAT: 0171-Z300049Size: 1 mgDry Ice: NoHazardous: No
CAT#:0171-Z300049Size:1 mg
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Description
Epidermal growth factor (EGF) is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites. EGF receptors are expressed in almost all types of tissues. Parietal endoderm, mature skeletal muscles, and hematopoietic tissues do not express the receptor. The EGF receptor, designated HER1, is a 170 kDa transmembrane glycoprotein with a length of 1186 amino acids. It is identical with a previously described glycoprotein called SA-7 (species antigen 7). The extracellular receptor domain has a length of 621 amino acids, including 11 glycosylated asparagine residues and 51 cysteine residues. This domain contains the EGF binding site and also binds mammalian TGF-alpha.
CAS Number
9000-83-3
Gene Name
EGF
Gene Aliases
EGF
Recombinant Rat Epidermal Growth Factor (EGF)
Gene ID
25313
Accession Number
P07522
Organism
Rat
Source
E. coli
Concentration
<1.0 EU/μg of recombinant protein as determined by the LAL method.
Purity
>95% as determined by SDS-PAGE
Solubility
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Function
The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.1 ng/mL, corresponding to a specific activity of ≥ 1 x 10^7 units/mg.
Molecular Weight
6.2
Shipping Conditions
Ambient Temperature
Storage Conditions
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Appearance
Lyophilized Powder
Formulation
Rat EGF was lyophilized from a 0.2 μm filtered solution in PBS pH 7.5.

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