Recombinant Mouse SHH

• Description:

  Mouse SHH is part of a small group of secreted proteins that are vital for development in both vertebrates and invertebrates. Mouse SHH shares 99% homology with the human gene for SHH. Sonic HedgeHog is a protein that is vital in guiding the early embryo; it has been associated as the major inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. SHH binds to the patched receptor to activate the transcription of target genes. Sonic HedgeHog also regulates another factor, the gli oncogene. Recombinant Mouse SHH is a single, non-glycosylated polypeptide chain of 19.8 kDa.

• CAS Number:

  9000-83-3

• Gene Name:

  SHH

• Gene Aliases:

  Sonic hedgehog protein, HHG-1,

• Gene ID:

  20423

• Accession Number:

  Q62226

• Organism:

  Mouse

• Source:

  E. coli

• Concentration:

  <1.0 EU/μg of recombinant protein as determined by the LAL method

• Purity:

  >95% as determined by SDS-PAGE

• Function:

  The ED50 of this protein, as measured by its ability to induce alkaline phosphatase production in mouse myoblast cell, was in the range of 500ng-3 ug/mL.

• Molecular Weight:

  19.8

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

• Appearance:

  Lyophilized Powder

• Formulation:

  Lyophilized from a 0.2 µm filtered solution in phosphate buffer and NaCl