Recombinant Mouse SHH

CAT: 0171-Z200617Size: 250 µgDry Ice: NoHazardous: No
CAT#:0171-Z200617Size:250 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mouse SHH is part of a small group of secreted proteins that are vital for development in both vertebrates and invertebrates. Mouse SHH shares 99% homology with the human gene for SHH. Sonic HedgeHog is a protein that is vital in guiding the early embryo; it has been associated as the major inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. SHH binds to the patched receptor to activate the transcription of target genes. Sonic HedgeHog also regulates another factor, the gli oncogene. Recombinant Mouse SHH is a single, non-glycosylated polypeptide chain of 19.8 kDa.
CAS Number
9000-83-3
Gene Name
SHH
Gene Aliases
Sonic hedgehog protein, HHG-1,
Gene ID
20423
Accession Number
Q62226
Organism
Mouse
Source
E. coli
Concentration
< 1.0 EU/μg of recombinant protein as determined by the LAL method.
Purity
>95% as determined by SDS-PAGE
Function
The ED50 of this protein, as measured by its ability to induce alkaline phosphatase production in mouse myoblast cell, was in the range of 500ng-3 ug/mL.
Molecular Weight
19.8
Shipping Conditions
Ambient Temperature
Storage Conditions
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Appearance
Lyophilized Powder
Formulation
Lyophilized from a 0.2 µm filtered solution in phosphate buffer and NaCl

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