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Recombinant Mouse CXCL4 (PF4)

CAT: 0171-Z200309Size: 1 mgDry Ice: NoHazardous: No
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CAT#:0171-Z200309Size:1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Platelet factor 4 (PF4/CXCL4) is an ELR− tetrameric, cationic chemokine that constitutes 25% of the protein in platelet α-granules. It is also found bound to the luminal vascular endothelial surface. Although platelets represent the primary source of PF4, it is also expressed at lower levels in other cells of the immune system including cultured T cells, monocytes, and endothelial and smooth muscle cells.
CAS Number
9000-83-3
Gene Name
PF4
Gene Aliases
C-X-C motif chemokine 4, PF-4
Recombinant Mouse Platelet Factor 4 (PF4)
Accession Number
Q9Z126
Organism
Mouse
Source
E. coli
Concentration
<1.0 EU/μg of recombinant protein as determined by the LAL method.
Purity
>95% as determined by SDS-PAGE
Solubility
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Function
Determined by its ability to inhibit human FGF-basic-dependent proliferation of NR6R-3T3 mouse fibroblasts. The ED50 for this effect is typically 5.0-15 μg/mL.
Molecular Weight
9
Shipping Conditions
Ambient Temperature
Storage Conditions
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Appearance
Lyophilized Powder
Formulation
Recombinant mouse PF4/CXCL4 was lyophilized from a 0.2 μm filtered concentrated (1.0 mg/mL) solution in 20 mM PB, 1.5M NaCl pH 7.4.

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