Recombinant Human TGFA

• Description:

  An EGF family member, TGF-A is a cytokine that is produced following the protease digestion of its trans-membrane precursor proTGF-A. TGF-A is expressed both in normal tissues during embryogenesis and in adult tissues, such as the brain, keratinocytes, and macrophages and at high levels in transformed and tumour cells.

• Gene Name:

  TGFA

• Gene Aliases:

  TGF-alpha, TGF-A, isoform CRA_d
  Recombinant Human Transforming Growth Factor Alpha (TGFA)

• Gene ID:

  7039

• Accession Number:

  P01135

• Organism:

  Human

• Source:

  E. coli

• Concentration:

  <1.0 EU/μg of recombinant protein as determined by the LAL method.

• Purity:

  >95% as determined by SDS-PAGE

• Solubility:

  Reconstitute at 100 μg/mL in sterile 10 mM Acetic Acid

• Function:

  Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED(50) for this effect is typically 0.1­0.4 ng/mL.

• Molecular Weight:

  5.8

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

• Appearance:

  Lyophilized Powder

• Formulation:

  Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA

• CAS Number:

  9000-83-3