Recombinant Human NRG4

• Description:

  Neuregulins (NDF, heregulin, GGF ARIA, or SMDF) are EGF-like growth and differentiation factors that signal through tyrosine kinase receptors of the ErbB family. The ErbB2 and ErbB4 receptors cooperate in transmission of neuregulin-1 signals in the heart, whereas ErbB2 and ErbB3 cooperate in neural crest cells.

• Gene Name:

  NRG4

• Gene Aliases:

  Pro-neuregulin-4, membrane-bound isoform, Pro-NRG4, NRG4
  Recombinant Human Neuregulin-4 (NRG4)

• Gene ID:

  145957

• Accession Number:

  Q8WWG1

• Organism:

  Human

• Source:

  E. coli

• Concentration:

  <1.0 EU/μg of recombinant protein as determined by the LAL method.

• Purity:

  >95% as determined by SDS-PAGE

• Solubility:

  A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

• Function:

  The ED(50) was determined by the phosphorylation of ErbB2 and ErbB4 receptors in CHO cells, and was found to be < 3.0 ng/mL, corresponding to a specific activity of 3.0 x 10^5 Units/mg.

• Molecular Weight:

  7

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

• Appearance:

  Lyophilized Powder

• Formulation:

  Recombinant Neuregulin-4 was lyophilized from a 0.2 μm filtered 20 mM PB, pH 6.0.

• CAS Number:

  9000-83-3