Recombinant Human Nodal

Recombinant Human Nodal

• Description: Nodal, also known as Nodal homolog, is a secreted protein member of the Transforming Growth Factor-beta (TGF-beta) superfamily. It is essential for the formation of mesoderm and axial patterning during early vertebrate embryonic development.
• CAS Number: 9000-83-3
• Gene Name: NODAL
• Gene Aliases: Nodal homolog
• Gene ID: 4838
• Accession Number: Q96S42
• Organism: Human
• Source: E. coli
• Concentration: <1.0 EU/μg of recombinant protein as determined by the LAL method
• Purity: >95% as determined by SDS-PAGE
• Solubility: Reconstitute in sterile 4mM HCl at 100µg/mL (containing at least 0.1% human or bovine serum albumin)
• Function: 100 ng/mL phosphorylation activity as determined by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells
• Molecular Weight: 13.1
• Shipping Conditions: Ambient Temperature
• Storage Conditions: The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
• Appearance: Lyophilized Powder
• Formulation: Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA (with BSA as a carrier protein)