Recombinant Human NAP-2 (PPBP) (CXCL7)

• Description:

  The chemokine neutrophil- activating peptide 2 (NAP-2) is a 70-amino acid residue polypeptide that is formed from platelet-derived precursors by proteolytic processing. Mature NAP-2 stimulates various effector functions of polymorphonuclear neutrophil granulocytes (PMN) including directed chemotactic migration, exocytosis of lysosomal enzymes and secondary granule contents, and up-regulation of adhesion receptors. NAP-2 has been assigned to a subfamily now termed alpha-chemokines. The alpha-chemokines contain four cysteine residues at highly conserved positions which enclose the core region of the molecules. The first two cysteines are separated by a single amino acid, forming a motif (CXC) that distinguishes the alpha-chemokine from the beta-chemokine subfamily, where these cysteines are in directly adjacent position.

• Gene Name:

  PPBP

• Gene Aliases:

  CXCL7, PBP (parent molecule), NAP2, CTAP-III (precursor), Platelet basic protein, C-X-C motif chemokine 7, Leukocyte-derived growth factor, LDGF, Macrophage-derived growth factor, MDGF, Small-inducible cytokine B7, SCYB7, TGB1, THBGB1
  Recombinant Human Neutrophil Activating Peptide-2 (PPBP)

• Gene ID:

  5473

• Accession Number:

  P02775

• Organism:

  Human

• Source:

  E. coli

• Concentration:

  <1.0 EU/μg of recombinant protein as determined by the LAL method.

• Purity:

  >95% as determined by SDS-PAGE

• Solubility:

  A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

• Function:

  Determined by its ability to chemoattract human monocytes using a concentration range of 2.0-40.0 ng/mL.

• Molecular Weight:

  8

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

• Appearance:

  Lyophilized Powder

• Formulation:

  Recombinant NAP-2 was lyophilized from a 0.2 μm filtered 20 mM PB,100 mM NaCl solution pH 7.5.

• CAS Number:

  9000-83-3