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Recombinant Human IL7

CAT: 0171-Z100979Size: 1 mgDry Ice: NoHazardous: No
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CAT#:0171-Z100979Size:1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
IL7 is secreted constitutively into the conditioned medium of adherent bone marrow stromal cells and thymic cells. Mouse and human keratinocytes have been shown also to express and secrete IL7.Human (152 amino acids; 17.4 kDa) and murine IL7 (129 amino acids) show 60 % sequence homology at the protein level. The human IL7 receptor is an integral strongly glycosylated membrane proteins of 76 kDa expressed on activated T-cells. This receptor has been designated as CD127. IL7 receptors are expressed on pre-B-cells and their progenitors. They are not expressed on mature B-cells. IL7 receptors are expressed also on bone marrow macrophages. Functional IL7 receptors are found on the cell surface of multiphenotypic, biphenotypic, and immature lymphoid progenitors of B- cells with the gene arrangement of the heavy immunoglobulin chain such as those observed in the germ line
CAS Number
9000-83-3
Gene Name
IL7
Gene Aliases
IL-7
Recombinant Human Interleukin-7 (IL7)
Gene ID
3574
Accession Number
P13232
Organism
Human
Source
E. coli
Concentration
<1.0 EU/μg of recombinant protein as determined by the LAL method.
Purity
>95% as determined by SDS-PAGE
Solubility
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Function
The ED50 as determined by dose-dependent stimulation of mouse lymphoma was <0.5ng/ml
Molecular Weight
17.7
Shipping Conditions
Ambient Temperature
Storage Conditions
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Appearance
Lyophilized Powder
Formulation
Lyophilized from a 0.2 μm filtered solution in sodium phosphate and NaCl

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