Recombinant Human IL28A

• Description:

  Interferon lambda-2, also known as Interleukin 28A, is an antiviral cytokine with immunomodulatory activity and is distantly related to the IL-10 family and type I interferons. IL28A along with IL29 and IL28B, are three closely related cytokines that form a cytokine gene cluster that use a distinct receptor system which induces antiviral defence signals. IL28A interacts with a heterodimeric class II cytokine receptor ligand and leads to the activation of the JAK/STAT signalling pathway, which ultimately leads to IFN-stimulated gene (ISG) and MHC class I antigen expression. It also plays a key role in antiviral defence in Toll-like receptors (TLR) and intestinal epithelia.

• Gene Name:

  IFNL2

• Gene Aliases:

  Zcyto20, Interleukin-28A, Interferon lambda-2

• Gene ID:

  282616

• Accession Number:

  Q8IZJ0

• Organism:

  Human

• Source:

  E. coli

• Concentration:

  <1.0 EU/μg of recombinant protein as determined by the LAL method

• Purity:

  >95% as determined by SDS-PAGE

• Solubility:

  A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

• Function:

  The ED50 is 10.0-50.0 ng/mL as determined by an anti-viral assay (using human hepatocellular carcinoma cells infected with EMC virus)

• Molecular Weight:

  19.5

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

• Appearance:

  Lyophilized Powder

• Formulation:

  Lyophilized from a 0.2 μm filtered solution containing no additives.

• CAS Number:

  9000-83-3