Recombinant Human IL17F

• Description:

  Interleukin 17F is a disulfide-linked homodimeric protein belonging to the IL-17 family of proinflammatory cytokines. Members belonging to this family share conserved features of cysteine-knot structures but differ in their N-terminal regions. The T-helper cell produced IL-17 family is inducible by IL15 and IL23, and IL17F induces T-cell and PBMC activation and proliferation. It also performs cartilage matrix turnover and angiogenesis prevention. Out of the IL-17 family, IL17F is the most homologous to IL17 and dimerizes in a way similar to that of nerve growth factors (NGF) and other neurotrophins.

• Gene Name:

  IL17F

• Gene Aliases:

  Cytokine ML-1

• Gene ID:

  112744

• Accession Number:

  Q96PD4

• Organism:

  Human

• Source:

  E. coli

• Concentration:

  <1.0 EU/μg of recombinant protein as determined by the LAL method

• Purity:

  >95% as determined by SDS-PAGE

• Solubility:

  A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

• Function:

  The ED50 is 8.0-40.0ng/mL as determined by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells

• Molecular Weight:

  15.2

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

• Appearance:

  Lyophilized Powder

• Formulation:

  Lyophilized from a 0.2μm filtered solution in 10mM sodium citrate buffer pH 3

• CAS Number:

  9000-83-3